Application and Effect
Thiol Quantification: DTNB is commonly used to assess the concentration of thiols in protein samples. This information is important for studying protein-protein interactions, protein folding, and enzymatic reactions involving thiol groups. The colorimetric response of DTNB allows for the quantification of thiols through spectrophotometric measurements. Evaluation of Redox State: DTNB has been employed to evaluate the redox state within cells, tissues, or biological samples. By measuring changes in thiol concentration, researchers can gain insights into oxidative stress, antioxidant activity, and redox signaling pathways. Enzyme Assays: The unique reactivity of DTNB with thiols makes it a valuable tool for enzyme assays. Many enzymes, such as glutathione-S-transferases, cystathionine-γ-lyase, and thioredoxin reductase, are thiol-dependent and can be quantified or assayed using DTNB. Stability Analysis of Metal-Sulfur Compounds: DTNB is used to assess the stability and reactivity of metal-sulfur complexes and metalloproteins. The compound can detect and quantify the presence of thiols involved in the chelation of metal ions, providing crucial information about metal-sulfur interactions and coordination chemistry.Product Packing:
Additional Information:
| Composition | C14H8N2O8S2 |
| Assay | 99% |
| Appearance | Yellow solid |
| CAS No. | 69-78-3 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |