5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt CAS:129541-41-9

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt is a chemical compound commonly used in laboratory research and diagnostics. It is often referred to as X-Gluc and is widely used as a substrate for the detection of beta-glucuronidase enzyme activity. When beta-glucuronidase is present, it cleaves the glucuronide bond in X-Gluc, resulting in the liberation of a blue dye called 5-bromo-4-chloro-3-indolyl. This reaction is commonly used to visually or spectrophotometrically detect the expression of the beta-glucuronidase enzyme in cells or tissues. The sodium salt form of X-Gluc improves its solubility in aqueous solutions, facilitating its use in laboratory assays. X-Gluc is mainly used in molecular biology research to study gene expression, promoter activity, and reporter gene assays. It can also be used to detect the presence of beta-glucuronidase-producing organisms, such as certain bacteria, in microbiological studies.

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Application and Effect

GUS detection: X-Gluc is cleaved by GUS enzyme into a blue insoluble compound known as 5-bromo-4-chloro-3-indole (X-Ind). This reaction allows for the visualization and quantification of GUS activity in cells and tissues. Gene expression studies: X-Gluc is used as a reporter molecule in gene expression studies. By fusing the GUS gene to a promoter of interest, researchers can determine the activity and spatial-temporal expression pattern of the promoter through the detection of GUS activity using X-Gluc. Transgenic plant analysis: The GUS reporter gene system is widely used in plant molecular biology. X-Gluc staining allows researchers to detect and study transgene expression patterns in plants. This helps in understanding gene regulation, tissue-specific expression, and developmental biology in plants. Genetic engineering: X-Gluc is used as a selectable marker in genetic engineering experiments. By linking the GUS gene to a foreign gene of interest, X-Gluc staining can be used to identify successful transformation and integration of the desired genes into the organism. Microbiology research: X-Gluc can be used to detect and identify GUS-producing bacteria. The enzyme GUS is found in many different bacterial species, and the staining with X-Gluc allows for the visualization and identification of GUS-positive bacteria in microbiological studies.

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Additional Information:

Composition C14H14BrClNNaO7
Assay 99%
Appearance White powder
CAS No. 129541-41-9
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

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