4-Aminophenyl-β-D-galactopyranoside CAS:5094-33-7

4-Aminophenyl-β-D-galactopyranoside is a synthetic compound that is similar to the substrate 3-Nitrophenyl-β-D-galactopyranoside (ONPG). It is used as a substrate for beta-galactosidase enzyme assays.When 4-aminophenyl-β-D-galactopyranoside is hydrolyzed by beta-galactosidase, it releases a yellow-colored compound called p-aminophenol. The activity of beta-galactosidase can be measured by quantifying the amount of p-aminophenol produced, typically through a colorimetric or spectrophotometric assay.This substrate is often used in conjunction with other derivatives and analogs of lactose to study beta-galactosidase activity, gene expression, enzyme inhibition or activation, and bacterial identification. The ability to detect and measure beta-galactosidase activity is crucial in many fields of research, including molecular biology, microbiology, and clinical diagnostics.  
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Application and Effect

Beta-galactosidase assay: APG can be used as a substrate to measure the activity of beta-galactosidase. This enzyme is commonly used as a reporter gene in molecular biology and genetic research. The assay helps determine the level of expression or activity of beta-galactosidase in various samples. Screening for enzyme inhibitors or activators: APG can be used to screen for compounds that inhibit or activate beta-galactosidase. By measuring the enzyme activity in the presence of different compounds, researchers can identify potential inhibitors or activators for further study. Bacterial identification: The presence of beta-galactosidase is often used as a marker to identify certain bacterial species. APG can be used in conjunction with other substrates or specific culture media to distinguish between different bacterial strains based on their ability to hydrolyze the substrate and produce a detectable product.

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Additional Information:

Composition C12H17NO6
Assay 99%
Appearance White powder
CAS No. 5094-33-7
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.
 

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