Factory Direct Supply 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS 4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide is a compound used in various biochemical studies, particularly for the detection and visualization of enzyme activity. It is a substrate that can be hydrolyzed by specific enzymes, resulting in the release of a colored or fluorescent product. This compound is commonly used in assays to detect the presence and activity of enzymes such as beta-galactosidase and beta-glucuronidase. These enzymes cleave the acetyl and glucosaminide groups from the substrate, leading to the formation of a blue or green chromophore. The unique structure of 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide allows for easy detection and quantification of enzyme activity. Its use in various experimental techniques, including histochemistry, immunohistochemistry, and cell-based assays, has contributed to a better understanding of enzyme functions.

Application and Effect

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) is a chromogenic substrate commonly used for the detection of beta-glucuronidase (GUS) activity. GUS is an enzyme found in various organisms, including bacteria, plants, and mammals. X-Gluc is frequently used in GUS reporter assays and molecular biology research. The main application of X-Gluc is in histochemical staining techniques, where it can visualize the expression and activity of the GUS enzyme. This substrate is cell-permeable and becomes hydrolyzed by GUS, resulting in the formation of a blue precipitate or insoluble product. This blue staining allows researchers to identify and localize GUS activity in cells, tissues, and whole organisms. X-Gluc can also be used in quantitative assays to measure GUS enzyme activity. The intensity of the blue color or the amount of product formed can be correlated with the level of GUS expression or its enzymatic activity. Additionally, X-Gluc has been employed in plant genetic research to study gene expression, promoter activity, and plant transformation. It has also been used in bacterial systems for cloning and detecting GUS fusion proteins.

Product Packing:

Additional Information:

Composition	C16H18BrClN2O6
Assay	99%
Appearance	White powder
CAS No.	4264-82-8
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

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