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5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) is a chemical compound commonly used in molecular biology and biochemistry applications. It is a chromogenic substrate for alkaline phosphatase enzymes. BCIP is often used in conjunction with nitroblue tetrazolium (NBT) as a substrate to detect alkaline phosphatase activity. When BCIP is dephosphorylated by alkaline phosphatase, a blue precipitate forms, allowing for the visualization of the enzyme's presence or activity. This compound is particularly useful in applications such as immunohistochemistry, in situ hybridization, and enzyme-linked immunosorbent assays (ELISAs) to detect the presence or localization of specific biomolecules or nucleic acids. The blue precipitate formed by BCIP provides a visible signal that assists in the identification and analysis of target molecules in experimental samples.

Application and Effect

Immunohistochemistry (IHC): BCIP is often used in IHC assays to visualize the presence or localization of specific antigens in tissue samples. The blue precipitate produced by BCIP allows for the identification of antigenic sites and can be used for qualitative or semi-quantitative analysis. In situ hybridization (ISH): BCIP is commonly employed in ISH techniques to detect and localize specific nucleic acid sequences, such as mRNA or DNA. The blue precipitate formed by BCIP indicates the presence of the target sequences and aids in the visualization of their distribution within cells or tissues. Enzyme-linked immunosorbent assays (ELISAs): BCIP is sometimes used in ELISAs to detect the presence or concentration of specific biomolecules, such as proteins or antibodies. By conjugating BCIP with an appropriate enzyme label and using alkaline phosphatase to catalyze the reaction, the blue color produced can indicate the presence and abundance of the biomolecule of interest.

Product Packing:

Additional Information:

Composition	C8H7BrClNNaO4P
Assay	99%
Appearance	White powder
CAS No.	102185-33-1
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.

5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

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