Application and Effect
Protein Purification: Avidin can be immobilized on a solid support, such as chromatography resin or magnetic beads, and used to capture biotinylated proteins or nucleic acids. This technique is known as avidin affinity chromatography, and it enables purification of specific target molecules in a highly specific manner. Biotinylated Probes: Avidin is often conjugated with enzymes, fluorophores, or other labels and used as a detection reagent in various assays. Biotinylated probes, such as antibodies or nucleic acid probes, can be detected by binding to avidin-linked detection molecules. This technique is commonly used in ELISA, immunohistochemistry, and nucleic acid hybridization assays. Targeted Drug Delivery: Avidin can be utilized as a carrier molecule to deliver drugs or therapeutic agents specifically to biotin receptor-expressing cells or tissues. By conjugating biotin to drugs or nanoparticles and linking them to avidin, targeted drug delivery systems can be created. Molecular Biology Techniques: Avidin-based systems are widely used in molecular biology research. For example, streptavidin, which has a similar binding affinity to biotin as avidin, is used in techniques such as DNA sequencing, PCR, and in situ hybridization, where it is paired with biotinylated probes or primers. Imaging and Diagnostics: Avidin can be labeled with radioisotopes, fluorescent dyes, or nanoparticles, making it a valuable tool in imaging techniques such as positron emission tomography (PET), fluorescence microscopy, and magnetic resonance imaging (MRI). Additionally, avidin-based systems are used in diagnostic tests, such as immunoassays, to detect the presence of specific biomarkers.Product Packing:
Additional Information:
| Composition | N.A. |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 1405-69-2 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |