b-D-Glucopyranoside,2-chloro-4-nitrophenyl 2-(acetylamino)-2-deoxy- CAS:103614-82-0

2-chloro-4-nitrophenyl-N-acetylglucosaminide is a chemical compound that belongs to the family of N-acetylglucosamine derivatives. It is commonly used as a substrate in enzymatic assays to measure the activity of certain enzymes, such as glycosidases and glycosyltransferases. The compound has a molecular structure consisting of a N-acetylglucosamine moiety, which is a derivative of glucose, and a 2-chloro-4-nitrophenyl group attached to the sugar molecule. The presence of the 2-chloro-4-nitrophenyl group makes this compound highly sensitive to enzymatic cleavage by glycosidases, resulting in the release of the 2-chloro-4-nitrophenol molecule. The amount of released 2-chloro-4-nitrophenol can be quantified through spectrophotometric measurements, allowing for the determination of enzyme activity. .  

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Application and Effect

Enzyme activity assays: It is commonly used as a substrate to measure the activity of glycosidases and glycosyltransferases. By monitoring the release of 2-chloro-4-nitrophenol, researchers can determine the enzyme activity and assess factors that may affect it, such as inhibitors or activators. High-throughput screening: The compound is used in drug discovery and development to screen large chemical libraries for potential enzyme inhibitors or activators. By measuring the activity of specific enzymes in the presence of different compounds, researchers can identify potential drug candidates. Glycobiology and glycosylation studies: 2-chloro-4-nitrophenyl-N-acetylglucosaminide is used to investigate glycosylation processes and study the role of glycosidases and glycosyltransferases in various cellular and biological processes. It helps researchers understand the function and regulation of glycosylation pathways. Biochemical research: The compound is widely used in biochemical research to study the kinetics, substrate specificity, and mechanisms of action of different enzymes involved in carbohydrate metabolism.

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Additional Information:

Composition C14H17ClN2O8
Assay 99%
Appearance White powder
CAS No. 103614-82-0
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.
 

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