Application and Effect
Effect: BCIP is a colorless compound that is converted into a blue-purple precipitate upon the action of alkaline phosphatase. This color change allows for visual detection and quantification of the enzyme's activity. Applications: Enzyme assays: BCIP is used in various biochemical assays to measure the activity of alkaline phosphatase. This includes detecting phosphatase activity in cell lysates, purified enzymes, or in vitro assays. Immunohistochemistry: BCIP can be used to visualize alkaline phosphatase-labeled antibodies in tissue sections or on cell surfaces. This technique is often used in immunostaining protocols. Blotting techniques: BCIP is used in Western blotting, Southern blotting, and Northern blotting to detect specific proteins or nucleic acids that have been labeled with alkaline phosphatase-conjugated probes. In situ hybridization: BCIP can be used to visualize gene expression patterns in tissues or cells through in situ hybridization methods. Diagnostic assays: BCIP is utilized as a substrate in various diagnostic kits for the detection of alkaline phosphatase. These kits are used in clinical laboratories for disease diagnosis and monitoring.Product Packing:
Additional Information:
| Composition | C8H7BrClNNaO4P |
| Assay | 99% |
| Appearance | Pale yellow powder |
| CAS No. | 102185-33-1 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |