Factory Direct 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine Salt Supplier CAS 6578-06-9

BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt CAS:6578-06-9

BCIP-Toluidine, also known as 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt, is a chemical compound commonly used in molecular biology and biochemistry research. It is primarily used as a substrate in enzyme-linked immunosorbent assays (ELISAs) or immunohistochemical staining techniques to detect alkaline phosphatase activity. When BCIP-Toluidine is cleaved by alkaline phosphatase, it undergoes a color change reaction, producing a blue-purple precipitate. This reaction allows for the visualization and quantification of alkaline phosphatase activity in samples. The intensity of the color can be measured spectrophotometrically and is directly proportional to the enzyme activity. The use of BCIP-Toluidine as a substrate is especially common in applications involving DNA or RNA labeling, such as in situ hybridization (ISH) or Southern and Northern blotting. It provides a reliable and sensitive method for detecting the presence of target nucleic acids in samples.

Application and Effect

BCIP-Toluidine (5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt) is a substrate used in molecular biology techniques to detect the presence of alkaline phosphatase activity. When used as a substrate, BCIP-Toluidine undergoes a color change reaction upon cleavage by alkaline phosphatase, resulting in the formation of a blue-purple precipitate. This color change allows researchers to visually detect and quantify alkaline phosphatase activity in various types of assays. The main application of BCIP-Toluidine is in enzyme-linked immunosorbent assays (ELISAs) and immunohistochemical staining techniques. It is often used to detect alkaline phosphatase-conjugated antibodies or alkaline phosphatase-labeled probes in these assays. BCIP-Toluidine is commonly utilized in molecular biology research for visualizing and quantifying gene expression, protein localization, and enzyme activity.

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Additional Information:

Composition	C8H6BrClNO4P.C7H9N
Assay	99%
Appearance	White powder
CAS No.	6578-06-9
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.

BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt CAS:6578-06-9

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