Application and Effect
Buffering: CHES is commonly used as a buffering agent to maintain a stable pH in biological experiments and enzymatic reactions. It is especially useful in applications requiring a pH range of 8.5 to 10. Electrophoresis: CHES is frequently used as a buffer in electrophoresis techniques, such as SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), to separate proteins based on their molecular weight. Enzyme assays: CHES is utilized in enzyme assays to maintain an optimal pH for enzyme activity. It helps to ensure that enzymatic reactions occur under controlled and reliable conditions. Cell culture media: CHES is sometimes included in cell culture media for various cell types as a pH-regulating component. It helps maintain physiological pH levels, which is critical for proper cell growth and function. Protein studies: CHES is often utilized in protein purification and protein crystallization experiments. Its buffering properties help maintain the stability and integrity of proteins during these processes.Product Packing:
Additional Information:
| Composition | C8H17NO3S |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 103-47-9 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |