Application and Effect
Calcium chelation: EGTA has a high affinity for calcium ions and can effectively bind to them, reducing the concentration of free calcium in a solution. This property makes EGTA useful in studying the role of calcium in various biological processes. Calcium buffer: EGTA is often used to create calcium-free or low calcium buffers for experiments. By chelating calcium, EGTA helps in maintaining a desired concentration of calcium ions in solution, allowing researchers to control calcium-dependent reactions. Enzyme activity modulation: Many enzymes require specific metal ions, including calcium, for their activity. EGTA can be used to modulate enzyme activity by chelating and removing these required metal ions from the reaction mixture. Cell dissociation: EGTA is useful in cell dissociation and tissue disaggregation processes. It helps to break cell-cell and cell-extracellular matrix interactions by chelating calcium-dependent adhesion molecules, leading to the detachment of cells. Calcium indicator studies: The ability of EGTA to chelate calcium ions is advantageous for calcium indicator studies. By controlling the concentration of free calcium ions with EGTA, researchers can accurately assess the role of calcium in intracellular signaling and other physiological processes. Molecular biology techniques: EGTA is utilized in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzyme assays. It helps to stabilize nucleic acids and proteins by preventing metal-ion mediated degradation. Cell culture: EGTA is commonly used in cell culture to maintain low levels of calcium to study calcium-dependent cellular processes accurately. It facilitates the removal of calcium from the growth media, allowing researchers to investigate the role of calcium in cell biology.Product Packing:
Additional Information:
| Composition | C14H24N2O10 |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 67-42-5 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |