Application and Effect
DNA/RNA Visualization: EtBr is most commonly used to visualize DNA and RNA in agarose gel electrophoresis. It intercalates between the base pairs of the nucleic acid molecules, causing them to fluoresce when exposed to UV light. This allows researchers to visualize and analyze the separation of DNA or RNA fragments based on their size or sequence. DNA Staining: EtBr can be used to stain DNA in various experimental procedures. It can be added to DNA samples, such as purified plasmids or PCR products, to verify their presence and concentration. The stained DNA can then be visualized using UV light or a gel imaging system. DNA Quantification: EtBr can be used for quantifying DNA concentration. By comparing the intensity of EtBr-stained DNA bands in an agarose gel with known standards of DNA concentration, the approximate amount of DNA in a sample can be determined. Fluorescent Microscopy: EtBr can be employed in fluorescence microscopy to stain and visualize DNA in living cells or tissues. It allows researchers to study DNA localization and dynamics within cells or observe DNA fragmentation in apoptotic cells.Product Packing:
Additional Information:
| Composition | C21H20BrN3 |
| Assay | 99% |
| Appearance | Red to dark purple powder |
| CAS No. | 1239-45-8 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |