Application and Effect
Effect: Gly-Pro p-nitroanilide p-toluenesulfonate salt is specifically designed to be a substrate for proline-specific peptidases. These enzymes cleave the peptide bond between glycine (Gly) and proline (Pro) residues, releasing p-nitroanilide (pNA) as a product. The pNA molecule absorbs light at a specific wavelength, allowing for the measurement of the released pNA and estimation of the enzyme activity. Application: Gly-Pro p-nitroanilide p-toluenesulfonate salt is used in various research areas, including biochemistry, enzymology, and drug discovery, for the following applications: Enzyme activity assays: It is widely used to measure the activity of proline-specific peptidases, including dipeptidyl peptidases (DPPs) and prolyl endopeptidases (PEPs). Screening enzyme inhibitors: Gly-Pro p-nitroanilide p-toluenesulfonate salt can be used to evaluate the inhibitory potential of compounds or drugs on proline-specific peptidase activity. Any decrease in the release of pNA indicates the presence of an inhibitor. Enzyme kinetics studies: By varying the concentration of Gly-Pro p-nitroanilide p-toluenesulfonate salt and measuring the rate of pNA release, enzyme kinetics parameters such as Km (Michaelis-Menten constant) and Vmax (maximum velocity) can be determined. Diagnostic applications: The activity of proline-specific peptidases can be relevant in diagnostic tests for various diseases and conditions, such as infections and cancers. Gly-Pro p-nitroanilide p-toluenesulfonate salt can be used as a tool to assess the activity levels of these enzymes in patient samples.Product Packing:
Additional Information:
| Composition | C20H24N4O7S |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 65096-46-0 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |