Application and Effect
pH buffering: HEPES is widely used as a buffering agent to maintain a stable pH in cell culture media and biological assays. It works effectively in the physiological pH range of 6.8 to 8.2, making it suitable for maintaining optimal conditions for biological processes. Cell culture: HEPES is commonly added to cell culture media to stabilize the pH level, providing a suitable environment for cell growth and maintaining cell viability. It helps to prevent pH fluctuations that could negatively impact cell behavior and experimental results. Enzyme assays: HEPES is often used as a buffer in enzymatic reactions due to its ability to maintain a specific pH. It helps in maintaining optimal enzymatic activity and stability, thereby enhancing the accuracy and reliability of enzyme assays. Protein studies: HEPES is employed in various protein-related experiments, including protein purification, protein crystallization, and protein structure analysis. It helps to stabilize the pH and maintain proper protein folding and stability during these experiments. Electrophoresis: HEPES finds application in gel electrophoresis techniques, such as SDS-PAGE and agarose gel electrophoresis. It is used as a buffering agent in the running buffer, providing a stable pH environment for the separation and analysis of biomolecules. Pharmaceutical formulations: HEPES is utilized in the formulation of pharmaceutical and biotechnological products. It helps to maintain the stability and integrity of active ingredients, ensuring their efficacy and shelf life.Product Packing:
Additional Information:
| Composition | C8H18N2O4S |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 7365-45-9 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |