Methylphenazinium methosulfate CAS:299-11-6

Methylphenazinium methosulfate (MPMS) is a redox-active compound that is commonly used as an electron carrier in various biochemical and biophysical studies. It is a salt consisting of a methylphenazinium cation (a heterocyclic compound) and a methosulfate anion. MPMS is often used as an alternative to traditional electron carriers, such as ferricyanide or phenazine ethosulfate, due to its stability and high solubility in water. It possesses good redox properties, which allow it to accept and transfer electrons during enzymatic reactions. One of the key applications of MPMS is in assays that involve the measurement of electron transfer or enzymatic activity. It is frequently used in conjunction with an enzyme system to monitor the transfer of electrons between different components. The reduction of MPMS can be detected spectrophotometrically, where its absorbance changes as a result of electron transfer processes. MPMS is also utilized in studies related to mitochondrial respiration and oxidative phosphorylation. It can act as an artificial electron acceptor, allowing researchers to investigate the functioning and regulation of these processes in various biological systems.  

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Application and Effect

Electron transfer and enzymatic activity: MPMS is used as an artificial electron acceptor in enzymatic assays to measure electron transfer and enzymatic activity. It can be reduced by donating electrons to enzymes and other redox-active molecules, allowing researchers to study the kinetics and efficiency of enzymatic reactions. Mitochondrial respiration and oxidative phosphorylation: MPMS is frequently used in studies focused on mitochondrial metabolism and energy production. It serves as an electron carrier in mitochondrial respiration, allowing researchers to measure electron flow and investigate various aspects of oxidative phosphorylation. Membrane potential measurements: MPMS can be used to measure changes in the membrane potential of cells. By monitoring the reduction and re-oxidation of MPMS, researchers can assess alterations in the electrochemical gradient across the cell membrane, which is important in processes such as ion transport, signaling, and cellular homeostasis. Assessment of antioxidant capacity: MPMS is used in antioxidant capacity assays to measure the ability of compounds or biological samples to scavenge free radicals and protect against oxidative stress. The reduction of MPMS is indicative of the antioxidant capacity of the tested substances. Redox-sensitive dyes: MPMS is frequently used as a redox-sensitive dye in various experimental setups. Its ability to undergo reversible oxidation and reduction reactions makes it suitable for monitoring redox changes in biological samples or cells.

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Additional Information:

Composition C14H14N2O4S
Assay 99%
Appearance Yellow crystalline powder
CAS No. 299-11-6
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.
 

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