Application and Effect
The effect of MOPS (3-(N-morpholino)propanesulfonic acid) is primarily related to its buffering capacity and ability to maintain a stable pH level. MOPS is a zwitterionic compound, meaning it contains both a positive and a negative charge, which allows it to act as an effective buffer in biological systems. One of the key applications of MOPS is in cell culture, where it is used to maintain the pH of the growth medium. Cells require a stable pH for optimal growth and function, and MOPS helps in buffering the medium and preventing pH fluctuations that can be detrimental to cell health. MOPS is also commonly used in molecular biology techniques such as DNA and RNA isolation, PCR (polymerase chain reaction), and gel electrophoresis. In these applications, MOPS helps to stabilize the pH of reaction mixtures and running buffers, ensuring accurate and reliable results. In protein analysis, MOPS can be used as a buffering agent in techniques such as protein purification, protein quantification, and protein electrophoresis. It helps to maintain the proper pH environment required for protein stability and activity during these procedures. Additionally, MOPS can be utilized in enzyme reactions and enzyme kinetics studies. Its buffering capacity allows for the maintenance of optimal pH conditions, which is crucial for enzyme activity and accurate kinetic measurements.Product Packing:
Additional Information:
Composition | C7H15NO4S |
Assay | 99% |
Appearance | White powder |
CAS No. | 1132-61-2 |
Packing | Small and bulk |
Shelf Life | 2 years |
Storage | Store in cool and dry area |
Certification | ISO. |