N,N'-Methylenebisacrylamide CAS:110-26-9

N,N'-Methylenebisacrylamide (MBAA) is a chemical compound commonly used in molecular biology and biochemistry applications. It is a crosslinking agent that is added to acrylamide gels to polymerize them and create a stable matrix for electrophoresis. Crosslinking refers to the formation of chemical bonds between polymer chains, and MBAA plays a crucial role in this process. When added to acrylamide monomers, MBAA links them together to form a three-dimensional network of polymers. This crosslinked structure enhances the stability and strength of the gel, allowing for the separation of biomolecules based on their size, charge, or other physical properties. MBAA is primarily utilized in techniques like polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), commonly used for protein and nucleic acid analysis. It helps in creating a uniform gel matrix through which the biomolecules can migrate during electrophoresis. Aside from its use in gel electrophoresis, MBAA also finds applications in other areas such as molecular imprinting, chromatography, and drug delivery systems. It is known for its ability to create stable and mechanically robust polymer networks, making it a valuable tool in research and analytical laboratories.

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Application and Effect

Crosslinking Effect: MBAA can link together acrylamide monomers to form crosslinked polymer networks. This crosslinking effect enhances the stability and strength of gels or polymer matrices. Gel Electrophoresis: One of the most common applications of MBAA is in gel electrophoresis techniques like polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). MBAA is added to acrylamide monomers to crosslink them and form a stable gel matrix, allowing the separation of biomolecules based on their size and charge during electrophoresis. Protein Analysis: MBAA is crucial in protein research and analysis. It allows for the separation of proteins by size and enables Western blotting, protein purification, and other protein characterization techniques. Nucleic Acid Analysis: MBAA is also used in nucleic acid analysis techniques like DNA sequencing, DNA fragment separation, and DNA footprinting. It provides a stable gel matrix that facilitates the separation of DNA fragments based on their size. Molecular Imprinting: MBAA is employed in molecular imprinting techniques, where a template molecule is incorporated into a polymer matrix. After removal of the template, the polymer matrix retains memory of the molecule's shape, allowing selective binding and recognition. Drug Delivery Systems: MBAA can be used to create crosslinked polymer networks with the ability to release drugs or other molecules in a controlled manner. It has applications in creating sustained-release formulations for pharmaceuticals, enhancing drug stability, and improving drug delivery efficiency.

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Additional Information:

Composition C7H10N2O2
Assay 99%
Appearance White powder
CAS No. 110-26-9
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.
 

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