Application and Effect
NSP-SA-NHS, also known as N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, is a compound commonly used as a thiol-specific crosslinking reagent in bioconjugation reactions. Its main effect is the formation of stable thioester bonds between thiol groups present on biomolecules, such as proteins or peptides. The application of NSP-SA-NHS is primarily in the field of protein modification and immobilization. Some of its key applications include: Protein labeling: NSP-SA-NHS is used to covalently attach labels, such as fluorescent dyes or biotin, to proteins or peptides. This allows for detection, identification, and tracking of the labeled biomolecules in various biological assays. Protein-protein interactions: NSP-SA-NHS can be used to crosslink interacting proteins to study protein-protein interactions. This is often used in techniques like co-immunoprecipitation or pull-down assays to identify binding partners or study protein complexes. Protein immobilization: NSP-SA-NHS allows for the covalent attachment of proteins or peptides onto solid surfaces, including agarose beads, magnetic beads, or microplates. This is useful in applications such as affinity purification, drug screening, or biosensor development. Surface modification: NSP-SA-NHS can be used to modify surfaces, such as glass slides or nanoparticles, with proteins or peptides, creating biomolecule-functionalized surfaces for various applications like diagnostics, drug delivery systems, or bio-sensing platforms.Product Packing:
Additional Information:
| Composition | C32H31N3O10S2 |
| Assay | 99% |
| Appearance | Yellow green powder |
| CAS No. | 199293-83-9 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |