Application and Effect
The effect of ONPG as a substrate is to be cleaved by the enzyme β-galactosidase, resulting in the release of a yellow product, o-nitrophenol. This color change can be measured spectrophotometrically, allowing for the quantification of β-galactosidase activity.The application of ONPG is primarily in the assessment of gene expression in molecular biology and microbiology research. It is commonly used to measure β-galactosidase activity as a reporter for gene expression studies, particularly in bacteria such as E. coli. The lacZ gene, which encodes β-galactosidase, is often used as a marker for gene expression analysis, as its expression can be induced by specific conditions or controlled by specific promoters.The ONPG assay provides a convenient and reliable method to assess the level of gene expression by measuring the activity of β-galactosidase. This assay is widely used in various applications, such as studying promoter activity, gene regulation, and protein-protein interactions. Additionally, it can be used to determine enzyme kinetics and evaluate the effects of mutations or treatments on enzyme activity.Product Sample
Product Packing:
Additional Information:
| Composition | C12H15NO8 |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 369-07-3 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |