Application and Effect
Enzyme activity assays: pNP-α-L-Fucopyranoside is commonly used as a synthetic substrate to measure the activity of α-L-fucosidase enzymes. The enzyme cleaves the glycosidic bond between the fucose sugar and the p-nitrophenyl group, resulting in the release of p-nitrophenol. The amount of p-nitrophenol produced can be quantified colorimetrically or spectrophotometrically, allowing the determination of enzyme activity. Enzyme kinetics studies: The hydrolysis of pNP-α-L-Fucopyranoside can be used to determine kinetic parameters, such as the Michaelis-Menten constant (Km) and the maximum velocity (Vmax) of α-L-fucosidase activity. These parameters provide valuable information about the enzyme's efficiency and affinity for the substrate. Inhibitor screening: pNP-α-L-Fucopyranoside can be used to screen and evaluate potential inhibitors of α-L-fucosidase. By measuring the enzyme activity in the presence and absence of different compounds, researchers can identify and characterize inhibitors that can modulate the enzyme's function.Product Packing:
Additional Information:
| Composition | C12H15NO7 |
| Assay | 99% |
| Appearance | White crystalline powder |
| CAS No. | 10231-84-2 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |