Application and Effect
Protein Digestion and Purification: Proteinase K is frequently used in protein purification processes to remove unwanted proteins or contaminants. It can effectively cleave peptide bonds and digest proteins, facilitating the isolation and purification of target proteins. Nucleic Acid Extraction: Proteinase K is routinely used in DNA and RNA extraction protocols. By breaking down proteins and removing nucleases, it helps in the isolation of high-quality DNA or RNA without degradation. Proteinase K can handle a variety of sample types, including blood, tissues, or cells. PCR Cleanup: Proteinase K is also useful in PCR cleanup procedures. It can remove unwanted enzymes, primers, nucleotides, or other impurities that could interfere with downstream applications. Proteinase K digestion helps in achieving clean PCR products suitable for further analysis. DNA Fingerprinting: In DNA fingerprinting or forensic analysis, Proteinase K is used to extract DNA from various biological samples, such as blood, saliva, or hair. Its ability to degrade proteins aids in breaking down cell membranes, releasing DNA for subsequent analysis. Histology and Immunohistochemistry: Proteinase K assists in antigen retrieval in histology and immunohistochemistry techniques. It can break down cross-linkages formed during sample fixation, allowing better access to antigens and improving the quality of staining in tissue sections. Enzyme Inactivation: Proteinase K is often used to inactivate enzymes. Its ability to degrade enzymes effectively prevents carryover contamination from previous reactions, making it a valuable tool in many molecular biology experiments. Protein Structure and Function Studies: Proteinase K is applied in research to study protein structure and function. By selectively cleaving peptide bonds at specific sites, it can help identify important regions or functional domains within proteins.Product Packing:
Additional Information:
| Composition | C29H27N2O12P |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 39450-01-6 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |