Application and Effect
Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate, also known as MESNa, has a specific effect and application in biochemistry and molecular biology. It is primarily used as a reducing agent for the protection of thiol (-SH) groups in proteins and peptides. MESNa serves as a powerful reducing agent, capable of converting disulfide bonds (-S-S-) in proteins into sulfhydryl (-SH) groups. This reduction process is essential in various scientific applications, including protein analysis, purification, and protein engineering. Some specific applications of MESNa include: Protein Denaturation: MESNa can be used to denature proteins by breaking disulfide bonds, thereby unfolding the protein structure and facilitating further analysis or protein purification. Reducing Protein Aggregation: MESNa can prevent protein aggregation by reducing disulfide bonds, ensuring protein stability and solubility in solution. Protein Labeling: MESNa can be used to introduce labels or modifications at specific cysteine residues in proteins by breaking the disulfide bonds and exposing the sulfhydryl (-SH) groups. Protein Refolding: MESNa is used to assist protein refolding after denaturation by reducing disulfide bonds and aiding in the correct formation of protein structures.Product Packing:
Additional Information:
| Composition | C12H20NNaO3S |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 40567-80-4 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |