Application and Effect
Tris-acetate (TRIS-Acetate) is a commonly used buffer in biological and biochemical experiments. It consists of a combination of tris(hydroxymethyl)aminomethane (Tris) and acetic acid, which acts as a pH regulator and stabilizer. The pH of a TRIS-Acetate buffer typically ranges from 7.4 to 8.4. The main effect of TRIS-Acetate is to maintain a stable pH, which is crucial for numerous biological and biochemical reactions. It works as a buffer by minimizing any significant changes in pH that may occur due to added acids or bases during experimental procedures. TRIS-Acetate finds various applications in molecular biology, biochemistry, and biotechnology: DNA and RNA Electrophoresis: TRIS-Acetate is commonly used as a running buffer in agarose and polyacrylamide gel electrophoresis. It provides a stable pH environment during the separation of DNA and RNA fragments based on their size. Protein Analysis: TRIS-Acetate buffers are used for protein electrophoresis, such as SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). It ensures protein stability and separation during the process. Enzyme Reactions: TRIS-Acetate buffers are frequently used in enzyme assays and studies. It provides the optimal pH range for various enzymatic reactions and helps maintain enzyme activity. Cell and Tissue Culture: TRIS-Acetate buffers are used in cell culture media to maintain the appropriate pH for cell growth and proliferation. It helps to maintain the physiological conditions required for cell viability.Product Packing:
Additional Information:
| Composition | C6H15NO5 |
| Assay | 99% |
| Appearance | White powder |
| CAS No. | 6850-28-8 |
| Packing | Small and bulk |
| Shelf Life | 2 years |
| Storage | Store in cool and dry area |
| Certification | ISO. |